Minnesota Microbiome Data Engine Wiki

Amplicon Sequencing

• Experimental Design
  • High-diversity samples
  • Low-diversity samples
• Sample Preparation
  • Sample Collection
  • DNA extraction
• Sequencing Library Preparation
  • Library Types
• OTU Clustering
  • Process
  • Running at MSI
  • Running on a Mac or Linux computer
• PICRUSt
  • Installing PICRUSt
  • Running PICRUSt

Shotgun Sequencing

Guidelines for shotgun metagenomic sequencing will be developed in the future.

The Minnesota Microbiome Data Engine Wiki is developed and maintained by

• Dan Knights Lab
• University of Minnesota Genomics Center
• University of Minnesota Informatics Institute